MYO1A Antibody
| Code | Size | Price |
|---|
| PSI-62-841-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Unconventional myosin-Ia, Brush border myosin I, BBM-I, BBMI, Myosin I heavy chain, MIHC, MYO1A, MYHL
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
MYO1A belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein envelops an actin core bundle that, by comparison, is static in structure.
Background References:
- D'Adamo,P., Pinna,M. Hum. Genet. 112 (3), 319-320 (2003)
- Hoshimaru,M., Fujio,Y. J. Biochem. 106 (3), 455-459 (1989)
- Durrbach,A., Collins,K. Proc. Natl. Acad. Sci. U.S.A. 93 (14), 7053-7058 (1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MYO1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human MYO1A.
NCBI Gene ID #:
4640
NCBI Official Name:
Unconventional myosin-Ia
NCBI Official Symbol:
MYO1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
118 kDa
Protein Accession #:
Q9UBC5
Protein GI Number:
13431715
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9UBC5
User NOte:
Optimal dilutions for each application to be determined by the researcher.