MED7 Antibody
| Code | Size | Price |
|---|
| PSI-56-144-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Mediator of RNA polymerase II transcription subunit 7, hMED7, Activator-recruited cofactor 34 kDa component, ARC34, Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9, Mediator complex subunit 7, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, MED7, ARC34, CRSP9
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
The activation of gene transcription is a multistep
process that is triggered by factors that recognize transcriptional
enhancer sites in DNA. These factors work with co-activators to
direct transcriptional initiation by the RNA polymerase II
apparatus. The protein encoded by this gene is a subunit of the
CRSP (cofactor required for SP1 activation) complex, which, along
with TFIID, is required for efficient activation by SP1. This
protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor-(TR-) associated proteins which interact
with TR and facilitate TR function on DNA templates in conjunction
with initiation factors and cofactors. Two transcript variants
encoding the same protein have been found for this gene. [provided
by RefSeq].
Background References:
- Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
- Sato, S., et al. Mol. Cell 14(5):685-691(2004)
- Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
- Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This MED7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human MED7.
NCBI Gene ID #:
9443
NCBI Official Name:
Mediator of RNA polymerase II transcription subunit 7
NCBI Official Symbol:
MED7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
O43513
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O43513
User NOte:
Optimal dilutions for each application to be determined by the researcher.