ROR1 Antibody

ProSci
Product Code: PSI-63-128
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-128-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis of ROR1 using rabbit polyclonal ROR1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2).
2 / 2
Flow cytometric analysis of NCI-H460 cells using ROR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ROR1 using rabbit polyclonal ROR1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR1 gene (Lane 2).
Flow cytometric analysis of NCI-H460 cells using ROR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, ROR1, NTRKR1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.
Background References:
  • Nomi, M., et al., Mol. Cell. Biol. 21(24):8329-8335 (2001).
  • Reddy, U.R., et al., Genomics 41(2):283-285 (1997).
  • Reddy, U.R., et al., Oncogene 13(7):1555-1559 (1996).
  • Masiakowski, P., et al., J. Biol. Chem. 267(36):26181-26190 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ROR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human ROR1.
NCBI Gene ID #:
4919
NCBI Official Name:
Tyrosine-protein kinase transmembrane receptor ROR1
NCBI Official Symbol:
ROR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
104 kDa
Protein Accession #:
Q01973
Protein GI Number:
118572711
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q01973
User NOte:
Optimal dilutions for each application to be determined by the researcher.