CACNA2D1 Antibody

ProSci
Product Code: PSI-63-598
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-598-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CACNA2D1 Antibody in mouse brain tissue lysates (35ug/lane)
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Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CACNA2D1 Antibody in mouse brain tissue lysates (35ug/lane)
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Voltage-dependent calcium channel subunit alpha-2/delta-1, Voltage-gated calcium channel subunit alpha-2/delta-1, Voltage-dependent calcium channel subunit alpha-2-1, Voltage-dependent calcium channel subunit delta-1, CACNA2D1, CACNL2A, CCHL2A, MHS3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CACNA2D1 is a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits.
Background References:
  • Williams,M.E., et.al., Neuron(1), 71-84 (1992)
  • Chaudhuri,D., et.al., J. Gen. Physiol. 129 (5), 385-401 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit, Rat
Immunogen:
This CACNA2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human CACNA2D1.
NCBI Gene ID #:
781
NCBI Official Name:
Voltage-dependent calcium channel subunit alpha-2/delta-1
NCBI Official Symbol:
CACNA2D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125 kDa
Protein Accession #:
P54289
Protein GI Number:
262527579
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P54289
User NOte:
Optimal dilutions for each application to be determined by the researcher.