INA Antibody

ProSci
Product Code: PSI-62-218
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-218-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of INA using rabbit polyclonal INA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of INA using rabbit polyclonal INA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INA gene (Lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
INA is a class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Background References:
  • Armstrong,R.A.,Eur. J. Neurol. 13 (5), 528-532 (2006)
  • Suzuki,T., Eur. J. Neurosci. 21 (2), 339-350 (2005)
  • Cairns,N.J., Am. J. Pathol. 164 (6), 2153-2161 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This INA (alpha internexin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the Central region of human INA (alpha internexin).
NCBI Gene ID #:
9118
NCBI Official Name:
Alpha-internexin
NCBI Official Symbol:
INA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q16352
Protein GI Number:
20141266
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q16352
User NOte:
Optimal dilutions for each application to be determined by the researcher.