PPEF2 Antibody

ProSci
Product Code: PSI-57-709
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-709-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody at 1:1000 at each lane.
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PPEF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody at 1:1000 at each lane.
PPEF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Serine/threonine-protein phosphatase with EF-hands 2, PPEF-2, PPEF2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. [provided by RefSeq].
Background References:
  • Kutuzov, M.A., et al. Biochem. Biophys. Res. Commun. 293(3):1047-1052(2002)
  • Ramulu, P., et al. Mol. Cell. Biol. 21(24):8605-8614(2001)
  • Sherman, P.M., et al. Proc. Natl. Acad. Sci. U.S.A. 94(21):11639-11644(1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PPEF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human PPEF2.
NCBI Gene ID #:
5470
NCBI Official Name:
Serine/threonine-protein phosphatase with EF-hands 2
NCBI Official Symbol:
PPEF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
O14830
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O14830
User NOte:
Optimal dilutions for each application to be determined by the researcher.