ADRBK1 Antibody
| Code | Size | Price |
|---|
| PSI-62-582-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2, ADRBK1, BARK, BARK1, GRK2
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Beta-adrenergic receptor kinase (ADRBK1), also known as GRK2, phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. ADRBK1 is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Heart failure is accompanied by severely impaired beta-adrenergic receptor (beta-AR) function. An important mechanism for the rapid desensitization of beta-AR function is agonist-stimulated receptor phosphorylation by the beta-AR kinase (beta-ARK1), an enzyme known to be elevated in failing human heart tissue. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Background References:
- Li, J., et al., J. Biol. Chem. 278(32):30219-30226 (2003).
- Wan, K.F., et al., J. Biol. Chem. 278(20):18658-18663 (2003).
- Yang, X.L., et al., World J. Gastroenterol. 9(4):800-803 (2003).
- Hagen, S.A., et al., Anesthesiology 98(2):343-348 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ADRBK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 633-660 amino acids from the C-terminal region of human ADRBK1.
NCBI Gene ID #:
156
NCBI Official Name:
Beta-adrenergic receptor kinase 1
NCBI Official Symbol:
ADRBK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
P25098
Protein GI Number:
126302521
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P25098
User NOte:
Optimal dilutions for each application to be determined by the researcher.