LRP6 Antibody

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ProSci
Product Code: PSI-62-147
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-147-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of LRP6 using rabbit polyclonal LRP6 Antibody (C-term T1546) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of LRP6 using rabbit polyclonal LRP6 Antibody (C-term T1546) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LRP6 gene (Lane 2).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with LRP6 Antibody (C-term T1546), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.
Background References:
  • Liu, G., et al., Mol. Cell. Biol. 23(16):5825-5835 (2003). Tamai, K., et al., Nature 407(6803):530-535 (2000). Brown, S.D., et al., Biochem. Biophys. Res. Commun. 248(3):879-888 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LRP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1560 amino acids from the C-terminal region of human LRP6.
NCBI Gene ID #:
4040
NCBI Official Name:
Low-density lipoprotein receptor-related protein 6
NCBI Official Symbol:
LRP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
180 kDa
Protein Accession #:
O75581
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O75581
User NOte:
Optimal dilutions for each application to be determined by the researcher.