ECE2 Antibody

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ProSci
Product Code: PSI-60-991
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-991-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. prostate section using ECE2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Western blot analysis in U251 cell line lysates (35ug/lane).
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Immunohistochemical analysis of paraffin-embedded H. prostate section using ECE2 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in U251 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Endothelin-converting enzyme 2, ECE-2, Methyltransferase-like region, 211-, Endothelin-converting enzyme 2 region, ECE2, KIAA0604
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta-amyloid peptide accumulation in brain. May also have methyltransferase activity.
Background References:
  • Lorenzo M.-N., et al. Biochim. Biophys. Acta 1522:46-52(2001).
  • Funke-Kaiser H., et al. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
  • Nagase T., et al. DNA Res. 5:31-39(1998).
  • Clark H.F., et al. Genome Res. 13:2265-2270(2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ECE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-579 amino acids from the Central region of human ECE2.
NCBI Gene ID #:
9718
NCBI Official Name:
Endothelin-converting enzyme 2
NCBI Official Symbol:
ECE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
100 kDa
Protein Accession #:
O60344
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O60344
User NOte:
Optimal dilutions for each application to be determined by the researcher.