HDAC9 Antibody

Product Code: PSI-55-625

Supplier: ProSci

Code	Size	Price

PSI-55-625-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Both anti-HDAC9 N-term and C-term Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

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Documents

Further Information

Additional Names:

Histone deacetylase 9, HD9, Histone deacetylase 7B, HD7, HD7b, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HDAC9, HDAC7, HDAC7B, HDRP, KIAA0744, MITR

Application Note:

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

Background:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.

Background References:

Petrie, K., et al., J. Biol. Chem. 278(18):16059-16072 (2003). David, D., et al., Genomics 81(5):489-503 (2003). Mahlknecht, U., et al., Biochem. Biophys. Res. Commun. 293(1):182-191 (2002). Zhou, X., et al., Proc. Natl. Acad. Sci. U.S.A. 98(19):10572-10577 (2001). Zhang, C.L., et al., J. Biol. Chem. 276(1):35-39 (2001).

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-533 amino acids from the C-terminal region of human HDAC9.

NCBI Gene ID #:

9734

NCBI Official Name:

Histone deacetylase 9

NCBI Official Symbol:

HDAC9

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

111 kDa

Protein Accession #:

Q9UKV0

Protein GI Number:

19865267

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Research Area:

Neuroscience,Signal Transduction

Swissprot #:

Q9UKV0

User NOte:

Optimal dilutions for each application to be determined by the researcher.