GJB5 Antibody

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ProSci
Product Code: PSI-58-180
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-180-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GJB5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GJB5 antibody detected the GJB5 protein (arrow).

Documents

Further Information

Additional Names:
Gap junction beta-5 protein, Connexin-311, Cx311, GJB5
Application Note:
For IHC-P starting dilution is: 1:50~100

For WB starting dilution is: 1:1000
Background:
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) such as GJB5 are thought to play crucial functional roles associated with these channels.
Background References:
  • Richard, G., et al., Nat. Genet. 20(4):366-369 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GJB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-119 amino acids from the Central region of human GJB5.
NCBI Gene ID #:
2709
NCBI Official Name:
Gap junction beta-5 protein
NCBI Official Symbol:
GJB5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
O95377
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O95377
User NOte:
Optimal dilutions for each application to be determined by the researcher.