PCDH7 Antibody

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ProSci
Product Code: PSI-56-347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-347-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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PCDH7 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH7 Antibdy for immunohistochemistry.
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Western blot analysis in A549 cell line lysates (35ug/lane).
PCDH7 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH7 Antibdy for immunohistochemistry.

Documents

Further Information

Additional Names:
Protocadherin-7, Brain-heart protocadherin, BH-Pcdh, PCDH7, BHPCDH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq].
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Huang, Y.T., et al. J. Clin. Oncol. 27(16):2660-2667(2009)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCDH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1039-1066 amino acids from the C-terminal region of human PCDH7.
NCBI Gene ID #:
5099
NCBI Official Name:
Protocadherin-7
NCBI Official Symbol:
PCDH7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
116 kDa
Protein Accession #:
O60245
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O60245
User NOte:
Optimal dilutions for each application to be determined by the researcher.