SCYL1 Antibody
| Code | Size | Price |
|---|
| PSI-62-735-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase, SCYL1, CVAK90, GKLP, NTKL, TAPK, TEIF, TRAP
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
SCYL1 forms multimers following transfection into COS-7 cells. SCYL1 forms a 300-kD trimer using crosslinking reagents. Biochemical analysis revealed no phosphorylation or autophosphorylation activity.The 707-amino acid SCYL1 variant, variant 2, localized to centrosomes during mitosis. During interphase, fluorescence-tagged variant 2 localized in the cytoplasm as well as centrosomes. However, at the beginning of mitosis, the fluorescence appeared as a pair of bright nuclear foci that followed centrosome localization throughout mitosis, while maintaining diffuse cytoplasmic labeling. Endogenous variant 2 in HeLa cells showed a similar staining pattern. Centrosomal localization was independent of microtubules.
Background References:
- Tang, Z., et al., Biochem. Biophys. Res. Commun. 324(4):1324-1332 (2004).
- Kato, M., et al., Genomics 79(6):760-767 (2002).
- Liu, S.C., et al., Biochim. Biophys. Acta 1517(1):148-152 (2000).
- van Asseldonk, M., et al., Genomics 66(1):35-42 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SCYL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the N-terminal region of human SCYL1.
NCBI Gene ID #:
57410
NCBI Official Name:
N-terminal kinase-like protein
NCBI Official Symbol:
SCYL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
Q96KG9
Protein GI Number:
74762671
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q96KG9
User NOte:
Optimal dilutions for each application to be determined by the researcher.