PCDHB12 Antibody

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ProSci
Product Code: PSI-56-353
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-353-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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PCDHB12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB12 Antibdy for immunohistochemistry.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
PCDHB12 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB12 Antibdy for immunohistochemistry.

Documents

Further Information

Additional Names:
Protocadherin beta-12, PCDH-beta-12, PCDHB12
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.
Background References:
  • Vanhalst, K., et al. FEBS Lett. 495 (1-2), 120-125 (2001) :
  • Wu, Q., et al. Genome Res. 11(3):389-404(2001)
  • Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
  • Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCDHB12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-795 amino acids from the C-terminal region of human PCDHB12.
NCBI Gene ID #:
56124
NCBI Official Name:
Protocadherin beta-12
NCBI Official Symbol:
PCDHB12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
Q9Y5F1
Protein GI Number:
13431382
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9Y5F1
User NOte:
Optimal dilutions for each application to be determined by the researcher.