Recoverin Antibody
| Code | Size | Price |
|---|
| PSI-58-189-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Recoverin, Cancer-associated retinopathy protein, Protein CAR, RCVRN, RCV1
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
Recoverin belongs to a high-affinity calcium-binding family that includes neuronal calcium sensor-1, visinin-like proteins (VILIPs), guanylate cyclase-activating proteins (GCAPs), and Kv-channel interacting proteins (KchIPs). Features common to this family include four calcium-binding EF-hand domains, and an N-terminal myristoylation sequence. This family of proteins has been implicated in a broad range of cellular signaling functions, including phototransduction and neurotransmitter release, lipid metabolism, gene expression, and ion channel regulation. Myristoylation, the post-translational addition of a fatty acid tail, has been shown to have functional significance for other calcium-binding protein family members. Recoverin is subject to the posttranslational modification of myristoylation. Binding of calcium to recoverin elicits a change in conformation that exposes the buried hydrophobic myristoyl moiety to interaction with cell membranes and other cellular proteins.
Background References:
- Wiechmann, A., et al., Curr. Eye Res. 26(1):25-32 (2003). Matsubara, S., et al., Br. J. Cancer 74(9):1419-1422 (1996). Yamaji, Y., et al., Int. J. Cancer 65(5):671-676 (1996). McGinnis, J.F., et al., Mamm. Genome 4(1):43-45 (1993). Wiechmann, A.F., et al., Exp. Eye Res. 56(4):463-470 (1993).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Recoverin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-200 amino acids from the C-terminal region of human Recoverin.
NCBI Gene ID #:
5957
NCBI Official Name:
Recoverin
NCBI Official Symbol:
RCVRN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P35243
Protein GI Number:
464600
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P35243
User NOte:
Optimal dilutions for each application to be determined by the researcher.