ASPH Antibody
| Code | Size | Price |
|---|
| PSI-56-642-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Aspartyl/asparaginyl beta-hydroxylase, Aspartate beta-hydroxylase, ASP beta-hydroxylase, Peptide-aspartate beta-dioxygenase, ASPH, BAH
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
This gene is thought to play an important role in calcium
homeostasis. The gene is expressed from two promoters and undergoes
extensive alternative splicing. The encoded set of proteins share
varying amounts of overlap near their N-termini but have
substantial variations in their C-terminal domains resulting in
distinct functional properties. The longest isoforms (a and f)
include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain
that hydroxylates aspartic acid or asparagine residues in the
epidermal growth factor (EGF)-like domains of some proteins,
including protein C, coagulation factors VII, IX, and X, and the
complement factors C1R and C1S. Other isoforms differ primarily in
the C-terminal sequence and lack the hydroxylase domain, and some
have been localized to the endoplasmic and sarcoplasmic reticulum.
Some of these isoforms are found in complexes with calsequestrin,
triadin, and the ryanodine receptor, and have been shown to
regulate calcium release from the sarcoplasmic reticulum. Some
isoforms have been implicated in metastasis.
Background References:
- Xue, T., et al. Hybridoma (Larchmt) 28(4):251-257(2009)
- Luu, M., et al. Hum. Pathol. 40(5):639-644(2009)
- Finotti, A., et al. BMC Mol. Biol. 9, 110 (2008) :
- Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ASPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human ASPH.
NCBI Gene ID #:
444
NCBI Official Name:
Aspartyl/asparaginyl beta-hydroxylase
NCBI Official Symbol:
ASPH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
86 kDa
Protein Accession #:
Q12797
Protein GI Number:
145559444
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q12797
User NOte:
Optimal dilutions for each application to be determined by the researcher.