BEST2 Antibody

ProSci
Product Code: PSI-63-854
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-854-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of BEST2 Antibody in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of BEST2 Antibody in MDA-MB435,MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma with BEST2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1, BEST2, VMD2L1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
BEST2 is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif.
Background References:
  • Zhang,Y., et.al, Mol. Vis. 16, 200-206 (2010)
  • Marsey,L.L. et.al, J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BEST2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-403 amino acids from the C-terminal region of human BEST2.
NCBI Gene ID #:
54831
NCBI Official Name:
Bestrophin-2
NCBI Official Symbol:
BEST2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q8NFU1
Protein GI Number:
38503353
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q8NFU1
User NOte:
Optimal dilutions for each application to be determined by the researcher.