CHML Antibody

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ProSci
Product Code: PSI-61-049
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-049-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells using CHML Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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4 / 5
Immunohistochemical analysis of paraffin-embedded M. cerebellum section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysate from HT-1080 cell line, using CHML Antibody at 1:1000.
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Immunohistochemical analysis of paraffin-embedded H. stomach section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using CHML Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded M. cerebellum section using CHML Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from HT-1080 cell line, using CHML Antibody at 1:1000.

Documents

Further Information

Additional Names:
Rab proteins geranylgeranyltransferase component A 2, Choroideremia-like protein, Rab escort protein 2, REP-2, CHML, REP2
Application Note:
For IHC-P starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Less effective than CHM in supporting prenylation of Rab3 family.
Background References:
  • Cremers F.P.M.,et al.Hum. Mol. Genet. 1:71-75(1992).
  • Kasper G.,et al.Gene 295:27-32(2002).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Gregory S.G.,et al.Nature 441:315-321(2006).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CHML antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 624-656 amino acids from the C-terminal region of human CHML.
NCBI Gene ID #:
1122
NCBI Official Name:
Rab proteins geranylgeranyltransferase component A 2
NCBI Official Symbol:
CHML
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
P26374
Protein GI Number:
47117837
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P26374
User NOte:
Optimal dilutions for each application to be determined by the researcher.