MSI1 Antibody

ProSci
Product Code: PSI-56-147
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-147-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from SH-SY5Y cell line, using MSI1 Antibody at 1:1000 at each lane.
2 / 3
MSI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysate from SH-SY5Y cell line, using MSI1 Antibody at 1:1000 at each lane.
MSI1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Background References:
  • Fan, L.F., et al. IntColorectal Dis 25(1):17-23(2010)
  • de Sousa Abreu, R., et al. J. Biol. Chem. 284(18):12125-12135(2009)
  • Murayama, M., et al. J. Gastroenterol. 44(3):173-182(2009)
  • Gotte, M., et al. J. Pathol. 215(3):317-329(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MSI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the N-terminal region of human MSI1.
NCBI Gene ID #:
4440
NCBI Official Name:
RNA-binding protein Musashi homolog 1
NCBI Official Symbol:
MSI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O43347
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
O43347
User NOte:
Optimal dilutions for each application to be determined by the researcher.