AOC3 Antibody

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ProSci
Product Code: PSI-63-509
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-509-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of CEM cells using AOC3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Antibody staining AOC3 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: human lung lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human colon carcinoma with AOC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells using AOC3 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Membrane primary amine oxidase, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, SSAO, Vascular adhesion protein 1, VAP-1, AOC3, VAP1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity.
Background References:
  • Lalor,P.F., et.al., J. Immunol. 169 (2), 983-992 (2002)
  • Salmi,M., et.al., Am. J. Pathol. 161 (6), 2255-2262 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AOC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 613-640 amino acids from the Central region of human AOC3.
NCBI Gene ID #:
8639
NCBI Official Name:
Membrane primary amine oxidase
NCBI Official Symbol:
AOC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q16853
Protein GI Number:
2501336
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q16853
User NOte:
Optimal dilutions for each application to be determined by the researcher.