MAP2 Antibody

ProSci
Product Code: PSI-60-917
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-917-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of MAP2 Antibody in MCF-7 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded mouse brain tissue reacted with MAP2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of MAP2 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded mouse brain tissue reacted with MAP2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Microtubule-associated protein 2, MAP-2, MAP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.
Background References:
  • Maddodi, N., et al. J. Biol. Chem. 285(1):242-254(2010)
  • Krishnan, C., et al. Am. J. Surg. Pathol. 33(11):1695-1704(2009)
  • Gambichler, T., et al. Am. J. Clin. Pathol. 131(5):710-714(2009)
  • Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAP2 antibody is generated from rabbits immunized with MAP2 recombinant protein.
NCBI Gene ID #:
4133
NCBI Official Name:
Microtubule-associated protein 2
NCBI Official Symbol:
MAP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
200 kDa
Protein Accession #:
P11137
Protein GI Number:
215274255
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P11137
User NOte:
Optimal dilutions for each application to be determined by the researcher.