PCDHGA3 Antibody

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ProSci
Product Code: PSI-57-156
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-156-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Ramos cell line lysates (35ug/lane).
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PCDHGA3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Ramos cell line lysates (35ug/lane).
PCDHGA3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Protocadherin gamma-A3, PCDH-gamma-A3, PCDHGA3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Wu, Q., et al. Genome Res. 11(3):389-404(2001)
  • Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
  • Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCDHGA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-311 amino acids from the Central region of human PCDHGA3.
NCBI Gene ID #:
56112
NCBI Official Name:
Protocadherin gamma-A3
NCBI Official Symbol:
PCDHGA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
101 kDa
Protein Accession #:
Q9Y5H0
Protein GI Number:
296439266
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q9Y5H0
User NOte:
Optimal dilutions for each application to be determined by the researcher.