MEF2C Antibody

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ProSci
Product Code: PSI-62-219
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-219-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
2 / 3
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.
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Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.

Documents

Further Information

Additional Names:
Myocyte-specific enhancer factor 2C, MEF2C
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.
Background References:
  • Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). Krainc, D., et al., Genomics 29(3):809-811 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This MEF2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from human MEF2C.
NCBI Gene ID #:
4208
NCBI Official Name:
Myocyte-specific enhancer factor 2C
NCBI Official Symbol:
MEF2C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q06413
Protein GI Number:
2500875
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q06413
User NOte:
Optimal dilutions for each application to be determined by the researcher.