PCDH1 Antibody

ProSci
Product Code: PSI-56-180
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-180-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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PCDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).
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PCDH1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).

Further Information

Additional Names:
Protocadherin-1, Cadherin-like protein 1, Protocadherin-42, PC42, PCDH1
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene.
Background References:
  • Koppelman, G.H., et al. Am. J. Respir. Crit. Care Med. 180(10):929-935(2009)
  • Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
  • Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
  • Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-206 amino acids from the N-terminal region of human PCDH1.
NCBI Gene ID #:
5097
NCBI Official Name:
Protocadherin-1
NCBI Official Symbol:
PCDH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
115 kDa
Protein Accession #:
Q08174
Protein GI Number:
215273864
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q08174
User NOte:
Optimal dilutions for each application to be determined by the researcher.