PCYT1A Antibody

ProSci
Product Code: PSI-56-255
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-255-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
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PCYT1A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western Blot at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
PCYT1A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Choline-phosphate cytidylyltransferase A, CCT-alpha, CTP:phosphocholine cytidylyltransferase A, CCT A, CT A, Phosphorylcholine transferase A, PCYT1A, CTPCT, PCYT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
PCYT1A controls phosphatidylcholine synthesis.
Background References:
  • Mostowska, A., et al. J. Med. Genet. (2010) In press :
  • Mostowska, A., et al. Eur. J. Oral Sci. 118(4):325-332(2010)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Hamster
Immunogen:
This PCYT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-54 amino acids from the N-terminal region of human PCYT1A.
NCBI Gene ID #:
5130
NCBI Official Name:
Choline-phosphate cytidylyltransferase A
NCBI Official Symbol:
PCYT1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
P49585
Protein GI Number:
166214967
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P49585
User NOte:
Optimal dilutions for each application to be determined by the researcher.