PCDH20 Antibody

ProSci
Product Code: PSI-55-965
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-965-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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PCDH20 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
PCDH20 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Protocadherin-20, Protocadherin-13, PCDH20, PCDH13
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain.
Background References:
  • Suzuki, S.T. Exp. Cell Res. 261(1):13-18(2000)
  • Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
  • Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
  • Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20.
NCBI Gene ID #:
64881
NCBI Official Name:
Protocadherin-20
NCBI Official Symbol:
PCDH20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
105 kDa
Protein Accession #:
Q8N6Y1
Protein GI Number:
544584765
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
Q8N6Y1
User NOte:
Optimal dilutions for each application to be determined by the researcher.