GPR142 Antibody

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ProSci
Product Code: PSI-55-742
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-742-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Probable G-protein coupled receptor 142, G-protein coupled receptor PGR2, GPR142, PGR2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
GPR142 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).
Background References:
  • Matsuo, A., et al. Biochem. Biophys. Res. Commun. 331(1):363-369(2005)
  • Fredriksson, R., et al. FEBS Lett. 554(3):381-388(2003)
  • Vassilatis, D.K., et al. Proc. Natl. Acad. Sci. U.S.A. 100(8):4903-4908(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GPR142 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the N-terminal region of human GPR142.
NCBI Gene ID #:
350383
NCBI Official Name:
Probable G-protein coupled receptor 142
NCBI Official Symbol:
GPR142
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
51 kDa
Protein Accession #:
Q7Z601
Protein GI Number:
83288229
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction,Growth Factor
Swissprot #:
Q7Z601
User NOte:
Optimal dilutions for each application to be determined by the researcher.