GPR45 Antibody

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ProSci
Product Code: PSI-56-301
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-301-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
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GPR45 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy for immunohistochemistry.
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Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
GPR45 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy for immunohistochemistry.

Documents

Further Information

Additional Names:
Probable G-protein coupled receptor 45, PSP24-1, PSP24-alpha, GPR45
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:10~50
Background:
This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.
Background References:
  • Salonen, J.T., et al. Am. J. Hum. Genet. 81(2):338-345(2007) Wu, C., et al. Proteomics 7(11):1775-1785(2007) Hillier, L.W., et al. Nature 434(7034):724-731(2005) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):957-964(2000) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):952-956(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45.
NCBI Gene ID #:
11250
NCBI Official Name:
Probable G-protein coupled receptor 45
NCBI Official Symbol:
GPR45
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q9Y5Y3
Protein GI Number:
82654940
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction,Growth Factor
Swissprot #:
Q9Y5Y3
User NOte:
Optimal dilutions for each application to be determined by the researcher.