APOL1 Antibody

ProSci
Product Code: PSI-62-853
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-853-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the detected the APOL1-BH3 Domain protein (arrow).
2 / 3
APOL1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the detected the APOL1-BH3 Domain protein (arrow).
APOL1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Apolipoprotein L1, Apolipoprotein L, Apo-L, ApoL, Apolipoprotein L-I, ApoL-I, APOL1, APOL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver.
Background References:
  • Li,Q., Clin. Chim. Acta 403 (1-2), 152-155 (2009)
  • Zhaorigetu,S., Autophagy(8), 1079-1082 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-171 amino acids from human APOL1.
NCBI Gene ID #:
8542
NCBI Official Name:
Apolipoprotein L1
NCBI Official Symbol:
APOL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
O14791
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity
Swissprot #:
O14791
User NOte:
Optimal dilutions for each application to be determined by the researcher.