GAD2 Antibody

Contact us
ProSci
Product Code: PSI-55-644
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-644-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody at 1:1000.
2 / 4
Western blot analysis in Jurkat cell line lysates (35ug/lane).
3 / 4
4 / 4
Confocal immunofluorescent analysis of GAD2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
❮❯

Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody at 1:1000.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of GAD2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
  • Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
  • Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human GAD2.
NCBI Gene ID #:
2572
NCBI Official Name:
Glutamate decarboxylase 2
NCBI Official Symbol:
GAD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q05329
Protein GI Number:
1352216
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience
Swissprot #:
Q05329
User NOte:
Optimal dilutions for each application to be determined by the researcher.