PYY Antibody

ProSci
Product Code: PSI-61-793
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-793-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PYY Antibody in MCF-7 cell line lysates (35ug/lane)
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PYY Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of PYY Antibody in MCF-7 cell line lysates (35ug/lane)
PYY Antibody IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Peptide YY, PYY, PYY-I, PYY
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PYY gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Background References:
  • Ueda, S.Y., et al. J. Endocrinol. 203(3):357-364(2009)
  • Shih, P.A., et al. J. Clin. Endocrinol. Metab. 94(11):4557-4566(2009)
  • Gratacos, M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (6), 808-816 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PYY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the C-terminal region of human PYY.
NCBI Gene ID #:
5697
NCBI Official Name:
Peptide YY {ECO:0000303|PubMed:2587421}
NCBI Official Symbol:
PYY
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
11 kDa
Protein Accession #:
P10082
Protein GI Number:
317373516
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience
Swissprot #:
P10082
User NOte:
Optimal dilutions for each application to be determined by the researcher.