GALC Antibody

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ProSci
Product Code: PSI-61-308
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-308-200ul200ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 2
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: Hela whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: Hela whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Galactocerebrosidase, GALCERase, 3.2.1.46, Galactocerebroside beta-galactosidase, Galactosylceramidase, Galactosylceramide beta-galactosidase, GALC
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
Background References:
  • Luzi P.,et al.Genomics 26:407-409(1995).
  • Sakai N.,et al.Biochim. Biophys. Acta 1395:62-67(1998).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Heilig R.,et al.Nature 421:601-607(2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This GALC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human GALC.
NCBI Gene ID #:
2581
NCBI Official Name:
Galactocerebrosidase
NCBI Official Symbol:
GALC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P54803
Protein GI Number:
229462868
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
P54803
User NOte:
Optimal dilutions for each application to be determined by the researcher.