Leptin Antibody

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ProSci
Product Code: PSI-62-142
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-142-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of hLep-R149 in MDA-MB231 cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of Leptin (LEP) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Leptin, Obese protein, Obesity factor, LEP, OB, OBS
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Leptin (LEP) is an adipocyte-secreted protein that regulates body fat and endocrine functions. Leptin is similar to the mouse obesity protein encoded by the obese gene (ob). In mouse studies, mutations in this gene are linked to severe and morbid obesity.
Background References:
  • Tank, J., et al., J. Clin. Endocrinol. Metab. 88(10):4955-4959 (2003).
  • Silha, J.V., et al., Eur. J. Endocrinol. 149(4):331-335 (2003).
  • Kazumi, T., et al., Metab. Clin. Exp. 52(9):1113-1116 (2003).
  • Mullington, J.M., et al., J. Neuroendocrinol. 15(9):851-854 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Leptin (LEP) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from the C-terminal region of human Leptin (LEP).
NCBI Gene ID #:
3952
NCBI Official Name:
Leptin
NCBI Official Symbol:
LEP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
P41159
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
P41159
User NOte:
Optimal dilutions for each application to be determined by the researcher.