TRPC5 Antibody

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ProSci
Product Code: PSI-57-257
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-257-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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TRPC5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
TRPC5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5, TRPC5, TRP5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1).
Background References:
  • Al-Shawaf, E., et al. Arterioscler. Thromb. Vasc. Biol. 30(7):1453-1459(2010)
  • Wong, C.O., et al. Pflugers Arch. 460(1):121-130(2010)
  • Miehe, S., et al. J. Biol. Chem. 285(16):12426-12434(2010)
  • Gross, S.A., et al. J. Biol. Chem. 284(49):34423-34432(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit
Immunogen:
This TRPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the N-terminal region of human TRPC5.
NCBI Gene ID #:
7224
NCBI Official Name:
Short transient receptor potential channel 5
NCBI Official Symbol:
TRPC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
111 kDa
Protein Accession #:
Q9UL62
Protein GI Number:
10720321
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
Q9UL62
User NOte:
Optimal dilutions for each application to be determined by the researcher.