PHGDH Antibody
| Code | Size | Price |
|---|
| PSI-61-613-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3
Application Note:
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Background:
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95)
catalyzes the transition of 3-phosphoglycerate into
3-phosphohydroxypyruvate, which is the first and rate-limiting step
in the phosphorylated pathway of serine biosynthesis, using
NAD+/NADH as a cofactor.
Background References:
- Du, H., et al. Reproduction 139(1):237-245(2010)
- Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)
- Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :
- Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Monkey
Immunogen:
This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH.
NCBI Gene ID #:
26227
NCBI Official Name:
D-3-phosphoglycerate dehydrogenase
NCBI Official Symbol:
PHGDH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
O43175
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
O43175
User NOte:
Optimal dilutions for each application to be determined by the researcher.