ASMT Antibody

ProSci
Product Code: PSI-61-665
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-665-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.
2 / 4
Western blot analysis of ASMT Antibody in 293 cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.
Western blot analysis of ASMT Antibody in 293 cell line lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT
Application Note:
For WB starting dilution is: 1:500-1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.
Background References:
  • Melke, J., et al. Mol. Psychiatry 13(1):90-98(2008)
  • Toma, C., et al. Mol. Psychiatry 12(11):977-979(2007)
  • Ross, M.T., et al. Nature 434(7031):325-337(2005)
  • Itoh, M.T., et al. Horm. Metab. Res. 35(3):153-157(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT.
NCBI Gene ID #:
438
NCBI Official Name:
Acetylserotonin O-methyltransferase
NCBI Official Symbol:
ASMT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
P46597
Protein GI Number:
1170276
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Neuroscience,Signal Transduction
Swissprot #:
P46597
User NOte:
Optimal dilutions for each application to be determined by the researcher.