ATP5D Antibody

ProSci
Product Code: PSI-58-754
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-754-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).

Western blot analysis in Jurkat cell line lysates (35ug/lane).

Further Information

Additional Names:
ATP synthase subunit delta, mitochondrial, F-ATPase delta subunit, ATP5D
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.
Background References:
  • Grimwood, J., et al. Nature 428(6982):529-535(2004)
  • Itoh, H., et al. Nature 427(6973):465-468(2004)
  • Cross, R.L. Nature 427(6973):407-408(2004)
  • Hong, S., et al. J. Bioenerg. Biomembr. 35(2):95-120(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ATP5D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the C-terminal region of human ATP5D.
NCBI Gene ID #:
513
NCBI Official Name:
ATP synthase subunit delta, mitochondrial
NCBI Official Symbol:
ATP5D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P30049
Protein GI Number:
584812
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P30049
User NOte:
Optimal dilutions for each application to be determined by the researcher.