GCAT Antibody
| Code | Size | Price |
|---|
| PSI-56-722-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase, GCAT, KBL
Application Note:
For WB starting dilution is: 1:1000
Background:
The degradation of L-threonine to glycine consists of a
two-step biochemical pathway involving the enzymes L-threonine
dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase.
L-Threonine is first converted into 2-amino-3-ketobutyrate by
L-threonine dehydrogenase. This gene encodes the second enzyme in
this pathway, which then catalyzes the reaction between
2-amino-3-ketobutyrate and coenzyme A to form glycine and
acetyl-CoA. The encoded enzyme is considered a class II
pyridoxal-phosphate-dependent aminotransferase. Alternate splicing
results in multiple transcript variants. A pseudogene of this gene
is found on chromosome 14.
Background References:
- Jacquot, C., et al. Anticancer Res. 22(4):2229-2235(2002)
- Edgar, A.J., et al. Eur. J. Biochem. 267(6):1805-1812(2000)
- Tressel, T., et al. J. Biol. Chem. 261(35):16428-16437(1986)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This GCAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-128 amino acids from the N-terminal region of human GCAT.
NCBI Gene ID #:
23464
NCBI Official Name:
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
NCBI Official Symbol:
GCAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
O75600
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
O75600
User NOte:
Optimal dilutions for each application to be determined by the researcher.