NDUFA12 Antibody

ProSci
Product Code: PSI-60-092
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-092-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A2058 cell line lysates (35ug/lane).

Western blot analysis in A2058 cell line lysates (35ug/lane).

Further Information

Additional Names:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12, 13 kDa differentiation-associated protein, Complex I-B172, CI-B172, CIB172, NADH-ubiquinone oxidoreductase subunit B172, NDUFA12, DAP13
Application Note:
For WB starting dilution is: 1:1000
Background:
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Background References:
  • Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)
  • Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003)
  • Murray, J., et al. J. Biol. Chem. 278(16):13619-13622(2003)
  • Hu, R.M., et al. Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NDUFA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human NDUFA12.
NCBI Gene ID #:
55967
NCBI Official Name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
NCBI Official Symbol:
NDUFA12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
Q9UI09
Protein GI Number:
12229870
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9UI09
User NOte:
Optimal dilutions for each application to be determined by the researcher.