CYP4F3 Antibody

ProSci
Product Code: PSI-63-566
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-566-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CYP4F3 Antibody in K562 cell line lysates (35ug/lane)
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Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CYP4F3 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Docosahexaenoic acid omega-hydroxylase CYP4F3, 20-hydroxyeicosatetraenoic acid synthase, 20-HETE synthase, 11413-, CYPIVF3, Cytochrome P450 4F3, Cytochrome P450-LTB-omega, Leukotriene-B(4) 20-monooxygenase 2, Leukotriene-B(4) omega-hydroxylase 2, CYP4F3, LTB4H
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
CYP4F3 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation.
Background References:
  • Kikuta,Y., et.al., FEBS Lett. 348 (1), 70-74 (1994)
  • Kikuta,Y., et,al., DNA Cell Biol. 17 (3), 221-230 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CYP4F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CYP4F3.
NCBI Gene ID #:
4051
NCBI Official Name:
Docosahexaenoic acid omega-hydroxylase CYP4F3
NCBI Official Symbol:
CYP4F3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
Q08477
Protein GI Number:
56757430
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q08477
User NOte:
Optimal dilutions for each application to be determined by the researcher.