PDE3B Antibody

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ProSci
Product Code: PSI-56-179
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-179-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of PDE3B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of PDE3B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B, CGIPDE1, CGIP1, Cyclic GMP-inhibited phosphodiesterase B, CGI-PDE B, PDE3B
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes; PDE3B may play a role in fat metabolism.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Yamano, Y., et al. Int. J. Cancer 126(2):437-449(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Hanson, M.S., et al. Am. J. Physiol. Heart Circ. Physiol. 295 (2), H786-H793 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDE3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-427 amino acids from the Central region of human PDE3B.
NCBI Gene ID #:
5140
NCBI Official Name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase B
NCBI Official Symbol:
PDE3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
124 kDa
Protein Accession #:
Q13370
Protein GI Number:
143811435
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q13370
User NOte:
Optimal dilutions for each application to be determined by the researcher.