EARS2 Antibody

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ProSci
Product Code: PSI-63-004
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-004-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-EARS2 Pab in HL60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with EARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Probable glutamate--tRNA ligase, mitochondrial, Glutamyl-tRNA synthetase, GluRS, EARS2, KIAA1970
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ?minimal set?of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.
Background References:
  • Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-523 amino acids from the C-terminal region of human EARS2.
NCBI Gene ID #:
124454
NCBI Official Name:
Probable glutamate--tRNA ligase, mitochondrial
NCBI Official Symbol:
EARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q5JPH6
Protein GI Number:
117949790
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q5JPH6
User NOte:
Optimal dilutions for each application to be determined by the researcher.