I23O2 Antibody

ProSci
Product Code: PSI-56-406
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-406-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining I23O2 in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
2 / 5
Western Blot at 1:4000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
3 / 5
4 / 5
Western blot analysis in K562 cell line lysates (35ug/lane).
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I23O2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Antibody staining I23O2 in Human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:4000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
I23O2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Indoleamine 2,3-dioxygenase 2, IDO-2, 11311-, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1, IDO2, INDOL1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).
Background References:
  • Cetindere, T., et al. Cell. Signal. 22(2):197-211(2010)
  • Huttunen, R., et al. Shock 33(2):149-154(2010)
  • Witkiewicz, A.K., et al. J. Am. Coll. Surg. 208(5):781-787(2009)
  • Metz, R., et al. Cancer Res. 67(15):7082-7087(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This I23O2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-295 amino acids from the C-terminal region of human I23O2.
NCBI Gene ID #:
169355
NCBI Official Name:
Indoleamine 2,3-dioxygenase 2
NCBI Official Symbol:
IDO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q6ZQW0
Protein GI Number:
215274147
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q6ZQW0
User NOte:
Optimal dilutions for each application to be determined by the researcher.