HDLBP Antibody

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ProSci
Product Code: PSI-63-936
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-936-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HDLBP Antibody in A2058, T47D cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HDLBP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of HDLBP Antibody in A2058, T47D cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HDLBP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Vigilin, High density lipoprotein-binding protein, HDL-binding protein, HDLBP, HBP, VGL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
High density lipoprotein-binding protein, also known as vigilin, is a 110-kD protein that specifically binds HDL molecules and may function in the removal of excess cellular cholesterol.
Background References:
  • Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009)
  • Zhou, J., et al. RNA 14(9):1773-1781(2008)
  • Rikova, K., et al. Cell 131(6):1190-1203(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This HDLBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 764-791 amino acids from the Central region of human HDLBP.
NCBI Gene ID #:
3069
NCBI Official Name:
Vigilin
NCBI Official Symbol:
HDLBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
141 kDa
Protein Accession #:
Q00341
Protein GI Number:
218511884
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q00341
User NOte:
Optimal dilutions for each application to be determined by the researcher.