BCAT2 Antibody

ProSci
Product Code: PSI-56-721
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-721-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of BCAT2 using rabbit polyclonal BCAT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.
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BCAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of BCAT2 using rabbit polyclonal BCAT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCAT2 gene.
BCAT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Branched-chain-amino-acid aminotransferase, mitochondrial, BCAT(m), Placental protein 18, PP18, BCAT2, BCATM, BCT2, ECA40
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Coles, S.J., et al. Biochemistry 48(3):645-656(2009)
  • Yennawar, N.H., et al. J. Biol. Chem. 281(51):39660-39671(2006)
  • Goto, M., et al. J. Biol. Chem. 280(44):37246-37256(2005)
  • Conway, M.E., et al. Biochemistry 43(23):7356-7364(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BCAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-336 amino acids from the C-terminal region of human BCAT2.
NCBI Gene ID #:
587
NCBI Official Name:
Branched-chain-amino-acid aminotransferase, mitochondrial
NCBI Official Symbol:
BCAT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
O15382
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
O15382
User NOte:
Optimal dilutions for each application to be determined by the researcher.