PNPLA4 Antibody

ProSci
Product Code: PSI-57-387
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-387-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in WiDr cell line lysates (35ug/lane).
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PNPLA4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in WiDr cell line lysates (35ug/lane).
PNPLA4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Patatin-like phospholipase domain-containing protein 4, Protein GS2, PNPLA4, DXS1283E, GS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y.
Background References:
  • Gao, J.G., et al. Mol. Genet. Metab. 96(4):253-260(2009)
  • Kienesberger, P.C., et al. J. Lipid Res. 50 SUPPL, S63-S68 (2009) :
  • Johansson, L.E., et al. PLoS ONE(4), E5327 (2009) :
  • Gao, J.G., et al. Biochem. Biophys. Res. Commun. 360(2):501-506(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PNPLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the C-terminal region of human PNPLA4.
NCBI Gene ID #:
8228
NCBI Official Name:
Patatin-like phospholipase domain-containing protein 4
NCBI Official Symbol:
PNPLA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P41247
Protein GI Number:
116242718
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P41247
User NOte:
Optimal dilutions for each application to be determined by the researcher.