CRAT Antibody

ProSci
Product Code: PSI-62-328
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-328-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CRAT antibody in CEM cell line lysates (35ug/lane)
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CRAT Antibody IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of CRAT antibody in CEM cell line lysates (35ug/lane)
CRAT Antibody IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Carnitine O-acetyltransferase, Carnitine acetylase, Carnitine acetyltransferase, CAT, CrAT, CRAT, CAT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
CRAT is carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments.
Background References:
  • Govindasamy,L., J. Struct. Biol. 146 (3), 416-424 (2004)
  • Jogl,G., Cell 112 (1), 113-122 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CRAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human CRAT.
NCBI Gene ID #:
1384
NCBI Official Name:
Carnitine O-acetyltransferase
NCBI Official Symbol:
CRAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
P43155
Protein GI Number:
215274265
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P43155
User NOte:
Optimal dilutions for each application to be determined by the researcher.