PGAM4 Antibody

ProSci
Product Code: PSI-57-004
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-004-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293,HepG2,Jurkat cell line lysates (35ug/lane).
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PGAM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in 293,HepG2,Jurkat cell line lysates (35ug/lane).
PGAM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Probable phosphoglycerate mutase 4, PGAM4, PGAM3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This intronless gene appears to have arisen from a retrotransposition event, yet it is thought to be an expressed, protein-coding gene. The encoded protein is a member of the phosphoglycerate mutase family, a set of enzymes that catalyze the transfer of a phosphate group from 3-phosphoglycerate to 2-phosphoglycerate.
Background References:
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
  • Marques, A.C., et al. PLoS Biol.(11), E357 (2005) :
  • Betran, E., et al. Mol. Biol. Evol. 19(5):654-663(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PGAM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 amino acids from the N-terminal region of human PGAM4.
NCBI Gene ID #:
441531
NCBI Official Name:
Probable phosphoglycerate mutase 4
NCBI Official Symbol:
PGAM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q8N0Y7
Protein GI Number:
26006838
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q8N0Y7
User NOte:
Optimal dilutions for each application to be determined by the researcher.