MGAT4C Antibody

ProSci
Product Code: PSI-56-542
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-542-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysates (35ug/lane).
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MGAT4C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in HepG2 cell line lysates (35ug/lane).
MGAT4C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C, N-acetylglucosaminyltransferase IV homolog, hGnT-IV-H, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVc, GlcNAc-T IVc, GnT-IVc, N-acetylglucosaminyltransferase IVc, UDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucosaminyltransferase IVc, MGAT4C
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri-and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity).
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Trynka, G., et al. Gut 58(8):1078-1083(2009)
  • Furukawa, T., et al. J. Hum. Genet. 44(6):397-401(1999)
  • Thomas, B.A. Br. J. Dermatol. 80 (6), 413 (1968) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Pig
Immunogen:
This MGAT4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-332 amino acids from the C-terminal region of human MGAT4C.
NCBI Gene ID #:
25834
NCBI Official Name:
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
NCBI Official Symbol:
MGAT4C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
Q9UBM8
Protein GI Number:
296437368
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9UBM8
User NOte:
Optimal dilutions for each application to be determined by the researcher.