PIP5K3 Antibody

ProSci
Product Code: PSI-62-704
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-704-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PIP5K3 using rabbit polyclonal PIP5K3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2).
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of PIP5K3 using rabbit polyclonal PIP5K3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, PIKFYVE, KIAA0981, PIP5K3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization.
Background References:
  • Sbrissa, D., et al., Mol. Cell. Biol. 24(23):10437-10447 (2004).
  • Yang, S.A., et al., J. Biol. Chem. 279(40):42331-42336 (2004).
  • Ikonomov, O.C., et al., J. Biol. Chem. 278(51):50863-50871 (2003).
  • Ikonomov, O.C., et al., Mol. Biol. Cell 14(11):4581-4591 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1561 amino acids from the Central region of human PIP5K3.
NCBI Gene ID #:
200576
NCBI Official Name:
1-phosphatidylinositol 3-phosphate 5-kinase
NCBI Official Symbol:
PIKFYVE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
237 kDa
Protein Accession #:
Q9Y2I7
Protein GI Number:
300669693
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9Y2I7
User NOte:
Optimal dilutions for each application to be determined by the researcher.